Real-time visualization of renal microperfusion using laser speckle contrast imaging

Significance: Intraoperative parameters of renal cortical microperfusion (RCM) have been associated with postoperative ischemia/reperfusion injury. Laser speckle contrast imaging (LSCI) could provide valuable information in this regard the advantage over current standard care being a non-contact and full-field technique. Aim: Our study aims to validate use LSCI for visualization RCM on ex vivo perfused human-sized porcine kidneys various models hemodynamic changes. Approach: A comparison was made between three perfusion measures: LSCI, total arterial blood flow (RBF), sidestream dark-field (SDF) different settings ischemia/reperfusion. Results: showed good correlation RBF reperfusion experiment (0.94 ± 0.02; p < 0.0001) short- long-lasting local ischemia (0.90 0.03; 0.0001 0.81 0.08; 0.0001, respectively). The decreased low situations due redistribution. SDF (0.81 0.10; superiority (0.54 0.22; 0.0001). Conclusions: is capable high spatial temporal resolutions. It can instantaneously detect deficits, which not possible care. Further development transplant surgery help clinical decision making.